The present research focuses on the use of 3D neural cell structures as resembling models of neuronal connectivity, to do so, during this study we employed cell models using Neurospheres (NS) and Cortical Organoids (CO) derived from human embryonic stem cells. The shape of these structures is spherical, which presents a problem specially when dealing with relatively big structures. This analysis could later be corroborated with Dr Cell, a program developed at the BioMEMS lab that can improve the analysis by the use of filters, thresholds, synchrony tests and spike bursts detection.